Rapid communication: TaqI restriction fragment length polymorphism at the porcine transporter associated with antigen processing 2 (TAP2) locus2
نویسندگان
چکیده
منابع مشابه
Rapid communication: TaqI restriction fragment length polymorphism at the porcine transporter associated with antigen processing 2 (TAP2) locus.
Source and Description of Probe. A 2.7-kb human cDNA clone for TAP2 was excised from the XbaI site of the plasmid pRSV.5neo (Bahram et al., 1991). Method of Detection. DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were for 16 to 20 h at 65°C (10% dextran sulfate, 7% SDS...
متن کاملRapid communication: TaqI restriction fragment length polymorphism at the porcine bone morphogenetic protein 6 (BMP6) locus.
Source and Description of Probe. A 1.1-kb human cDNA clone for bone morphogenetic protein 6 (BMP6) was excised from the clone U2-7 (Celeste et al., 1990). Method of Detection. Genomic DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and akaline vacuum-transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20...
متن کاملRapid communication: StuI restriction fragment length polymorphism at the porcine bone morphogenetic protein 5 (BMP5) locus.
Source and Description of Probe. A 2.2-kb human cDNA clone for bone morphogenetic protein 5 (BMP5) was excised from the clone U2-16 (Celeste et al., 1990).
متن کاملRapid communication: a TaqI restriction fragment length polymorphism in the ovine collagenase gene.
Polymorphism. Tag1 restriction fragment length polymorphism (RFLP) in the collagenase (CLG) of sheep. Source and Description of Probe. A 2.05-kb cDNA insert was excised from a pSP64 clone using HindIII and SmaI. The insert contains the complete coding sequence of the human fibroblast collagenase gene, as described by Angel et al. (1987). Method of Detection. Prehybridization and hybridization s...
متن کاملRapid communication: restriction fragment length polymorphism at the porcine superoxide dismutase locus.
PoZymorphism. An RFLP was detected in the superoxide dismutase 2 (SOD2) locus of swine using the restriction enzyme StuI. Source and Description of Probe. An 827-bp human cDNA clone for SOD2 was excised from the EcoRI site of plasmid phMnSOD4 (Xiang et al., 1987). Method of Detection. DNA was isolated from blood, digested with StuI, separated by agarose gel electrophoresis, and transferred ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 1994
ISSN: 0021-8812,1525-3163
DOI: 10.2527/1994.723798x